一株厌氧嗜热杆菌生长及发酵特性的初步研究

The Growth and Fermentation Characteristics of Thermoanaerobacterium saccharolyticum

摘要:

论文对筛选并鉴定为Thermoanaerobacterium saccharolyticum菌株的生长、底物利用情况、产物生成以及酒精耐受性进行了研究。结果表明, 该菌在以甘露糖、葡萄糖和木糖为碳源时生长较好, 同时能够较好的利用木聚糖和木薯淀粉; 最适底物浓度为15 g/L; 不同的葡萄糖: 木糖比例对其生长无显著影响; 能耐受的培养基最高初始酒精浓度为3% (V/V)。在5 g/L的木聚糖、木糖和木薯淀粉培养基中发酵60 h后, 产物主要有乙醇、乳酸和乙酸, 乙醇产量分别为0.824、0.867和0.916 g/L。

Abstract:

This paper studied on the growth, fermentation characteristics and ethanol tolerance of the strain identified as Thermoanaerobacterium saccharolyticum. The result showed that mannose, glucose and xylose were preferable to other carbon sources for its growth, while xylan and cassava starch could be consumed by the strain. Optimum concentration of substrate was 15 g/L. The ratio of glucose: xylose did not show significant influence on the growth. The highest tolerance on ethanol was 3% (V/V). Cultured on the medium of 5 g/L xylan, xylose and cassava starch for 60 h separately, lactic acid, ethanol and acetic acid were detected, and the ethanol concentration was 0.824, 0.867, 0.916 g/L, respectively.