表面张力曲线法测定生物表面活性剂的浓度

Determination of the Concentration of Biosurfactants Using a Surface Tension Curve Method

摘要:

介绍一种利用表面张力曲线测定生物表面活性剂发酵液浓度的方法, 通过测定不同浓度鼠李糖脂标准溶液的表面张力, 制作浓度和表面张力的x-y散点图, 再根据希斯科夫斯基经验公式利用Origin软件做x-y散点图的拟合曲线, 得到该公式的相关参数, 然后利用该经验公式, 通过测定稀释后待测发酵液的表面张力, 可求得原发酵液的鼠李糖脂浓度。和常用的方法相比, 该方法具有快速、简单、准确和测定成本低的优点。

关键词:鼠李糖脂, 生物表面活性剂, 表面张力, 浓度测定

Abstract:

In this study, we present the development of a new method for biosurfactants detection. A relation between surface tension and concentration of rhamnolipid was plotted in an x-y scatter by Origin using empirical formula. So the concentration of crude fermentation fluid was determined measuring surface tension of the diluted fermentation fluid with the fitted equation. This method is rapid, simple, accurate and low-cost compared to currently used methods.

Key words:Rhamnolipid, Biosurfactants, Surface tension, Concentration determination