科微学术
微生物学通报
2025年4月23日 周三
广东省粤港关键领域重点突破项目(No. 2008A030700003); 广东省省部产学研合作引导项目(No. 2009B090300299); 广东省科技计划项目(No. 2007A032400003)
LI An-Hua
1. South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong 510650, China; 2. Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China; 3. Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou, Guangdong 510070, China; 4. Guangdong Open Laboratory of Applied Microbiology, Guangzhou, Guangdong 510070, China; 5. Graduate School of Chinese Academy of Sciences, Beijing 100049, ChinaSUN Guo-Ping
2. Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China; 3. Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou, Guangdong 510070, China; 4. Guangdong Open Laboratory of Applied Microbiology, Guangzhou, Guangdong 510070, ChinaXU Mei-Ying
2. Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China; 3. Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou, Guangdong 510070, China; 4. Guangdong Open Laboratory of Applied Microbiology, Guangzhou, Guangdong 510070, China介绍一种利用表面张力曲线测定生物表面活性剂发酵液浓度的方法, 通过测定不同浓度鼠李糖脂标准溶液的表面张力, 制作浓度和表面张力的x-y散点图, 再根据希斯科夫斯基经验公式利用Origin软件做x-y散点图的拟合曲线, 得到该公式的相关参数, 然后利用该经验公式, 通过测定稀释后待测发酵液的表面张力, 可求得原发酵液的鼠李糖脂浓度。和常用的方法相比, 该方法具有快速、简单、准确和测定成本低的优点。
In this study, we present the development of a new method for biosurfactants detection. A relation between surface tension and concentration of rhamnolipid was plotted in an x-y scatter by Origin using empirical formula. So the concentration of crude fermentation fluid was determined measuring surface tension of the diluted fermentation fluid with the fitted equation. This method is rapid, simple, accurate and low-cost compared to currently used methods.
李安华,孙国萍,许玫英. 表面张力曲线法测定生物表面活性剂的浓度[J]. 微生物学通报, 2010, 37(7): 1083-1087
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