生物信息学法筛选志贺氏菌疫苗候选抗原

Screening Candidate Antigens for Shigella Vaccine by Bioinformatics Methods

摘要:

从GOLD基因组在线数据库中下载志贺氏菌S. flexneri 2a301、S. flexneri 5b8401和S. sonnei Ss046株的全基因组序列, 通过SignalP、PSORT-B、Cell-Ploc、TMHMM、Phobius、LipoP和PRED-TMBB等生物信息学工具, 筛选可能成为疫苗组份的表面抗原。结果分别从3株志贺氏菌的全基因组中得到了96、158和107个ORF, 它们分别编码一系列的脂蛋白、β-桶型跨膜蛋白或分泌性蛋白, 组成了一个疫苗候选抗原的信息库。上述ORF编码的蛋白在3株志贺氏菌中呈现良好的同源性, 有63种候选抗原在同源性分析中被发现同时存在于3株志贺氏菌中。说明生物信息学方法可以作为高通量筛选志贺氏菌疫苗候选抗原的辅助工具, 且为志贺氏菌新疫苗研制提供了大量有用的数据信息。

Abstract:

The bioinformatics analysis tools of SignalP, PSORT-B, Cell-Ploc, TMHMM, Phobius, LipoP and PRED-TMBB were used to screen putative surface-exposed or exported proteins for vaccine candidate antigens among three Shigella genome sequences of Shigella flexneri 2a strain 301, S. flexneri 2a strain 5b8401 and Shigella sonnei strain Ss046. As results, 96, 158 and 107 ORFs, putatively coding a series of lipoproteins, β-barrel outer membrane proteins and secreted proteins, respectively, were identified from these genome sequences. Among these ORFs, a total of 63 ORFs were conserved in all of these three genomes. In conclusion, bioinformatics methods could be used as an assistant tool in screening candidate antigens for the development of Shigella vaccines. The results might provide an useful basis for further studies of novel Shigella vaccines.