新疆达坂盐湖沉积土壤嗜盐细菌的定向富集与多样性分析

The Selective Enrichment and Diversity Analysis of Halophilic Bacteria in Sedimental Sample from Daban Salt Lake in Xinjiang

摘要:

采集新疆达坂盐湖的沉积土壤样品, 以选择性富集培养获得的嗜盐细菌基因组DNA为模板, 扩增16S rRNA基因, 在此基础上构建嗜盐细菌的16S rRNA基因文库, 随机挑选文库中的100个阳性克隆子进行群落结构多样性分析。16S rRNA基因序列分析结果表明: 100个克隆分属于细菌域9个属的27个种, 其中芽孢杆菌属(Bacillus)为优势菌群(48%), 喜盐芽孢杆菌属(Halobacillus)(14%)、盐单胞菌属(Halomonas)(13%)为次优势菌群。分析的阳性克隆子中, 10个克隆子与GenBank中已报道16S rRNA基因序列的相似性在88.80%到96.90%之间, 可能代表新属或新种。研究结果表明, 新疆达坂盐湖沉积土壤的富集培养物中存在种类较为丰富的嗜盐细菌。

Abstract:

The genomic DNA isolated from enriched culture of sedimentary soil samples from Daban Salt Lake in Xinjiang was used as PCR template to amplify 16S rRNA gene and the library carrying 16S rRNA genes of halophilic bacteria was constructed, 100 clones of the library were selected randomly and sequenced for molecular systematic analysis. Phylogenetic analysis indicated that 100 selected clones were clustered into twenty-seven species of nine genera, a lineage of the domain bacteria. Among them, Bacillus was the most dominant genus as represents 48% of the clones, followed by Halobacillus (14%) and Halomonas (13%). Furthermore, ten clones may be potential novel species or genera. The results show that the halophilic bacteria population diversity is abundant in enriched culture of sedimentary soil from Daban Salt Lake in Xinjiang.