UV与NTG复合诱变选育咪唑立宾高产菌

Breeding of a High Mizoribine-producing Strain by Composite Mutation with UV and NTG

摘要:

为选育咪唑立宾的高产菌株, 对咪唑立宾产生菌Eupenicillum sp. E-0509-2的孢子分别进行了紫外线照射(UV)、亚硝基胍(NTG)和UV + NTG复合诱变处理筛选突变株。结果表明试验所确定的咪唑立宾产生菌的孢子诱变适宜条件为: 将孢子悬液在电磁搅拌下, 经紫外线照射处理60 s后, 以3.0 g/L NTG处理20 min。经发酵筛选试验, 获得了一株遗传性状稳定、高产咪唑立宾的诱变菌株Eupenicillum sp. E-UN41, 其咪唑立宾产量较出发菌株提高13倍。

Abstract:

Eupenicillum sp. E-0509-2 was used as an original strain to carry out mutagenetic breeding adopting UV, NTG and UV + NTG, respectively. The spore suspension of E-0509-2 was treated by UV irradiation for 60 seconds, followed with 3.0 g/L NTG for 20 minuites under magnetic stirring. After further screening experiments on fermentation, a mutant Eupenicillum sp. E-UN41 which was able to produce mizoribine with high yield and could be stably passed on genetics was eventually found. The Mizoribine yield of Eupenicillum sp. E-UN41 was enhanced more than 13 times higher than that of the original strain.