微重力诱变大肠杆菌快速生长突变株特性

Mutagenesis of E. coli in Microgravity

摘要:

利用旋转培养装置处理大肠杆菌, 筛选生长曲线发生变化、提前进入对数期的突变菌株, 对菌株进行基因芯片的表达谱分析和质谱分析, 研究微重力条件下微生物的生理代谢变化和对微重力条件的适应机制。结果发现突变菌株有114个差异表达基因, 其中99个基因表达上调。表达上调基因主要集中在ABC转运系统、糖代谢、三羧酸代谢、磷酸转移酶系统、核酸代谢、脂类代谢等方面。质谱分析从蛋白水平上验证了这个结果。表明经过微重力处理可以筛选到生长加快的菌株, 生长加快是菌株相关代谢水平上调的结果。空间微重力通过对微生物生长代谢相关基因的影响来使菌株适应空间环境。

Abstract:

Variation in gene expression of Escherichia coli (E. coli), treated to micro-gravity, is analyzed to discover the change of microbial metabolism and the adaptation mechanism in microgravity using Microarray Gene Expression Profiles and Mass Spectrometry Analysis. In this article the strain was cultured in a rotator for several days and the mutant with advanced logarithmic phase in growth curve was screened and selected for Microarray Gene Expression Profiles and Mass Spectrometry Analysis. One hundred and fourteen differentially expressed genes are shown in Microarray Gene Expression Profiles of the mutant, and among them 99 were up-regulated expressions. The up-regulated expressions mainly appeared in ABC transporter, sugar metabolism, 3-carboxylic acid metabolism, phosphotransferase system, nucleic acid metabolism, lipid metabolism, etc. The result was verified through protein analysis with mass spectrometry. Suggesting that microgravity processing may accelerate microbial growth, and the advanced growth of the strain resulted from the up-regulated gene expression. The strain can adapt to spatial environments by affecting genes related to microbial metabolism.