食源性变形杆菌簇致病菌的检测

Detection of Food Borne Proteeae Pathogens

摘要:

为建立食源性变形杆菌簇致病菌的检测方法, 对富集培养基、选择性平板分离培养基和生化特性进行了筛选比较。结果表明, 肠杆菌富集肉汤培养基更有利于变形杆菌簇致病菌的检出, 平板分离培养基可用EMB琼脂培养基和SS琼脂培养基(或麦康凯琼脂培养基), 初筛方法为革兰氏反应和苯丙氨酸脱氨酶实验, 最后用其他生化反应进行确认。该方法检出率高, 检测范围较宽, 可为建立变形杆菌簇致病菌的检验标准提供实验依据。

Abstract:

To establish a detection method of food borne Proteeae pathogens, we compared different enrichment broths and different selective agars on Proteeae pathogen growth, correlated with the biochemical characteristics of the pathogens. The results showed that EE enrichment broth was more efficient than GN broth, and the selective agars were EMB agar plus SS agar (or MacConkey agar). The preliminary screen methods were Gram-stain reaction and phenylalanine deaminase reaction. The Proteeae pathogens were finally confirmed by other biochemical tests. This method was sensitive and had a broad scope of detection, could provide experimental basis for new standard.