枯草芽孢杆菌BS2对葡萄灰霉病菌抑菌机制的初步探索

Preliminary Exploration for Inhibitory Mechanism of Bacillus subtilis Strain BS2 to Botrytis Cinerea

摘要:

对葡萄灰霉病菌的生防枯草芽孢杆菌BS2菌液成分及胞外蛋白的抑菌机制进行了初步研究, BS2对葡萄灰霉病菌具有较好的拮抗作用, 其菌液成分和胞外蛋白经20°C-120°C处理后, 抑菌效果存在差异。BS2的菌液成分及胞外蛋白对灰霉病菌的产孢、萌发和菌丝的生长等方面均具有较好的抑制作用, 且对灰霉病菌菌丝的原生质有囊泡和颗粒化现象。由此分析, BS2抑菌活性物质是多种成分共同作用的结果, 抑菌物质中含有对温度敏感的高分子蛋白质, 且抑菌机制也是从多方面共同起作用。

Abstract:

The Bacillus subtilis strain 2 (BS2) was measured to antagonistic mechanism by using face to face culturing. The antagonistic effect of BS2 to Botrytis cinerea was obvious. Growth inhibition rate had significant difference between bacteria culture fluid and extracellular proteins at various temperatures (20°C-120°C). But the activity effect of extracellular proteins of BS2 was sensitive to temperature. Bacteria culture fluid and extracellular proteins produced from BS2 had a good antibacterial effect on B. cinerea mycelial growth, spores bearing and germination. And Treated with bacteria culture fluid and extracellular proteins produced from BS2, protoplast from the hyphae became abnormal. Overall, this study indicates that the BS2 active substance is composed of various components and has multiple antagonistic mechanism.