甘露寡糖对罗非鱼幼鱼肠道微生物的影响

Effects of Mannan-oligosaccharides on Intestinal Microorganisms of Tilapia Larvae

摘要:

分两个实验考察了饲料中添加自制酵母甘露寡糖、外购甘露寡糖和黄霉素对奥尼罗非鱼幼鱼[(l.00 ± 0.04) g]肠道菌群的影响。传统菌落计数结果表明, 不同的添加剂都能明显地改变幼鱼肠道中的乳酸杆菌和大肠杆菌的数量及比例, 其中0.5%自制酵母甘露寡糖使乳酸杆菌的数量增加10.77% (P < 0.05), 大肠杆菌的数量减少14.80% (P < 0.05), 使乳酸杆菌与大肠杆菌的比例提高到0.9720。DGGE图谱分析表明, 自制酵母甘露寡糖、外购甘露寡糖、黄霉素的加入都改变了罗非鱼幼鱼原有的肠道微生物的微生态环境, 其中自制酵母甘露寡糖对罗非鱼幼鱼肠道微生物的微生态影响最大。

Abstract:

The effects of mannan-oligosaccharides and flavomycin on the intestinal microorganisms of Tilapia larvae [(l.00 ± 0.04) g] were studied. The results showed that the number and ratio of Lactobacillus and E. coli in the intestinal tract of Tilapia larvae were obviously affected by three different additives including self-made yeast mannan-oligosaccharides, purchased mannan-oligosaccharides and flavomycin with different concentrations by plate count method. Among these additives, 0.5% self-made yeast mannan-oligosaccharides increased the Lactobacillus by 10.77% (P < 0.05), but reduce E. coli by 14.80% (P < 0.05), which increased the ratio of lactobacillus and E. coli to 0.9720. Furthermore, the Denatured Gradient Gel Electrophoresis (DGGE) fingerprints demonstrated that the original intestinal microbial microecology of Tilapia larvae was changed by all additives, among the three additives, self-made yeast mannan-oligosaccharides had the most significant influence on the intestinal microorganisms of Tilapia larvae.