一种副溶血性弧菌显色培养基的应用

Application of HKM Vibrio parahemolyticus Chromogenic Medium

摘要:

副溶血性弧菌(Vibrio parahemolyticus)是一种在海产品中广泛存在的嗜盐性细菌, 也是重要的食源性致病菌。传统检验方法检测周期长、特异性低, 为提高检测效率、缩短检测周期, 开发出一种副溶血性弧菌显色培养基(HKMVPM), 与国外同类产品KHVPM和TCBS在灵敏度、特异性及检测效果方面进行了比较和初步评价。结果显示, HKMVPM显色培养基和KHVPM显色培养基均具有较好的选择性和特异性, 这3种培养基对天然污染样品的分离率高低顺序为HKMVPM > KHVPM > TCBS。显色培养基具有高检出率及高特异性的特点, 有良好的应用前景。

Abstract:

Vibrio parahemolyticus is a saltophilic and important food-borne pathogen which widely exists in all kinds of seafood. The general method costs more time and has low specificity in isolating and identificating of V. parahemolyticus. In order to enhance the efficiency and shorten the detection periods, a new chromogenic medium (HKMVPM) was designed in this study. The sensitivity, specificity and detection efficiency of HKMVPM were studied and compared with that of KHVPM and TCBS by detecting the reference strains and natural samples. The results showed that the selectivity and specificity of HKMVPM were same as that of KHVPM. The detection efficiency of HKMVPM was better than that of KHVPM. The detection efficiency of KHVPM was better than that of TCBS. V. parahemolyticus chromogenic medium shows a high degree of higher sensitivity and specificity and has a good prospect in applying.