氰戊菊酯降解菌FDB的分离鉴定及其生长特性

Isolation, Identification and Growth Characteristics of a Fenvalerate Degradation Bacterium

摘要:

从长期受农药污染的农田土壤中分离筛选到一株降解氰戊菊酯杀虫剂的细菌菌株FDB。经形态和生理生化特征鉴定以及对16S rDNA序列进行同源性比较, 将该菌株鉴定为铜绿假单胞菌Pseudomonas aeruginosa。FDB能以氰戊菊酯杀虫剂为唯一碳源生长, 在30°C培养5 d对100 mg/L氰戊菊酯异构体的降解率分别达到69.06% (SR + RS)和64.32% (SS + RR)。FDB的最适生长条件为: 温度35°C, 初始pH值7.0, 250 mL摇瓶装液量75 mL。采用超声波方法破碎菌体细胞, 得到粗酶液。胞内和胞外粗酶液对氰戊菊酯异构体的降解试验表明, FDB的氰戊菊酯降解酶属于胞内蛋白组分。

Abstract:

Strain FDB, a fenvalerate-degrading bacterium, was isolated from some long-term pesticide-contaminated agricultural soils. The strain was identified as Pseudomnas aeruginosa based on its morphological and physiological properties, and 16S rDNA sequence analysis. Strain FDB grew in the medium supplemented with fenvalerate as its sole carbon source and degraded fenvalerate isomers at the concentration of 100 mg/L at the degradation rate from 69.06% (SR + RS) to 64.32% (SS + RR) in 5 days shaking at 30°C. The optimal growth was obtained under 35°C, pH 7.0 and 75 mL medium content 250 mL flask through shaking. The crude fenvalerate-degrading enzyme solution from cellular disruption was prepared by sonication, the activity of fenvalerate-degradation was associated with the intracellular fractions.