De-odorizing microbe F468 was used to reduce N nutrient losses and enhance the innocuous process of chicken manures. From its 1st to 80th subculture cycle, F468 showed stable ability to reduce NH3 emission from chicken manures. The total N nutrient losses were reduced by 79% by the addition of F468, compared with that without F468. Moreover, F468 could accelerate the innocent process of chicken manures by reducing Ascaris eggs and Fecal coliforms. Without adding F468, it took 25 days and 20 days for the dead rate of Ascaris eggs and the number of Fecal coliforms in chicken manure to reach the national standard of NY884-2004, respectively. While adding F468, it took 15 days and 10 days for the dead rate of Ascaris eggs and the number of Fecal coliforms to reach the national standard, respectively.