一株絮凝剂产生菌的筛选鉴定及培养条件

Screening and Identifying of a Kind of Flocculants-producing Bacteria and Its Cultural Conditions

摘要:

从淤泥中筛选到1株高效微生物絮凝剂产生菌JX18, 经过对其形态特征、生理生化特性、以及16S rRNA 序列分析初步鉴定为荧光假单胞菌。该菌培养40 h进入稳定期, 48 h时絮凝活性达到最大。进一步研究表明: 菌株JX18最适培养基初始pH值7.0, 培养温度30oC, 摇床转速160 r/min, 最佳碳源和氮源分别为葡萄糖和蛋白胨。最适培养条件下, 所产絮凝剂对高岭土悬浮液的絮凝活性达到90.56%。

Abstract:

A new kind of high flocculants-producing bacteria JX18 was isolated from sludge samples. It was identified as a Pseudomonas fluorescens based on its morphological, physiological characteristics and the partial sequences of its 16S rRNA. The bacteria reached stationary phase after culturing 40 h, and had a maximum ?occulating activity after culturing 48 h. The further investigation indicated that the optimum flocculants-producing conditions obtained were initial pH 7.0, temperature 30oC, rotation speed 160 r/min, glucose and peptone were chosen as the optimum carbon and nitrogen sources. Optimum cultivation conditions, the flocculation efficiency toward a gaolin clay suspension reached 90.56%.