新疆棉田土壤固氮菌遗传多样性分析

Genetic Diversity Analysis of Nitrogen-fixing Bacteria in Cotton Soil of the South Xinjiang

摘要:

利用ERIC-PCR和16S rDNA全序列测定方法, 研究了新疆棉田土壤中分离获得的58株固氮菌的遗传多样性及系统发育。采用平均连锁法(UPGMA)分析ERIC-PCR的聚类结果表明在Watson距离为0.65左右时可以将供试菌株分为9个大群。选取ERIC-PCR各群中代表菌株进行16S rRNA全序列测定分析, 结果表明这些菌株分别属于Enterobacter、Bacillus、Acinetobacter、Pseudomonas、Serratia和Yersinia 6个属。

Abstract:

To research the effect of nitrogen-fixing bacteria on grow of cotton, we used the technique of ERIC-PCR and 16S rDNA sequencing analysis to study genetic diversity of 58 nitrogen-fixing bacteria isolated from Tarim cotton soils in Xinjiang province. Cluster analysis was used for ERIC-PCR results of these strains by UPGMA. The results showed that the tested strains formed 9 clusters when the Waston distance was about 0.65. The typic strains of 9 clusters were identified as Enterobacter, Bacillus, Acinetobacter, Pseudomonas, Serratia, Yersinia by 16S rRNA sequencing analysis.