益生乳酸菌的纸片扩散法药敏性试验评价

Evaluation of Agar Disc Diffusion Method for Determining Antibiotic Susceptibility of Lactic Acid Bacteria Strains by a Selected Culture Medium

摘要:

乳酸菌菌株的药敏性评价是目前国际上益生乳酸菌安全性评价的首要内容, 然而用于乳酸菌药敏性测试的相关国际通引标准还未出台。本文就药敏试验使用的培养基进行了探讨, 并从中选取了合适的商品化培养基——RCA用于乳酸菌的药敏性测试。选取的培养基适合目前市场上常用益生菌的生长, 经方法优化后可得到满意的结果, 为今后制定针对乳酸菌的抗菌药物敏感性试验执行标准提供了一定依据。随后采用纸片扩散法对分属于4个属的9株乳酸菌进行了19类51种抗生素的敏感性测定, 较全面地了解了这些菌株的药敏谱。

Abstract:

The evaluation of antibiotic susceptibility of lactic acid bacteria (LAB) is a major part of the safety of probiotic foods. However, there is no international standard method of the susceptibility testing of LAB till now. We investigated 6 culture media and selected a proper commercial one from them. We optimized the agar disc diffusion method and assayed 9 strains of LAB encompassing the genera Lactobacillus, Bifidobacterium, Streptococcus and Lactococcus for susceptibility to 51 antibiotics on RCA agar. The optimized method proved to be an applicable technique for antibiotic susceptibility testing of LAB. This contributes to the establishment of a standardized procedure for antibiotic susceptibility testing of LAB.