野生黄芩内生真菌的分离鉴定及抗菌活性筛选

Isolation, Identification and Inhibitory Activity Screening of Endophytic Fungi from Wild Plants of Scutellaria baicalensis Georgi

摘要:

从野生黄芩的根、茎、叶中分离出97株内生真菌, 其中33株来自生长期植株, 64株来自成熟期植株。经分类鉴定, 隶属于38个属, 优势属为Alternaria, 成熟期的属种多样性高于生长期。将其中的95株内生真菌对14种指示菌进行抑菌试验, 发现其中91株内生真菌对一种或多种指示菌具有抑菌活性, 占分离菌株总数的95.8%。野生黄芩内生真菌对大肠杆菌CGMCC1.1103、枯草芽孢杆菌CGMCC1.769、蜡样芽孢杆菌ATCC1361、致病性大肠埃希氏菌ATCC49105、白色假丝酵母ATCC10231、小孢拟盘多毛孢CNUMB2PM01等6个指示菌菌株抑菌效果较好; 对单核细胞增生李斯特菌ATCC27708、普通变形杆菌ATCC33420、肠炎沙门氏菌ATCC14208、副溶血型弧菌ATCC27519、宋内氏痢疾杆菌ATCC51060、九州镰孢霉CNUMB2FK01等6个指示菌菌株抑菌效果较差; 对深红酵母CGMCC2.282和金黄色葡萄球菌ATCC12600没有抑制作用。

Abstract:

Ninety-seven strains of endophytic fungi were isolated from the roots, stems and leaves of natural Scutellaria baicalensis Georgi, in which, thirty-three from the living stage and sixty-four from the riping stage. They were classified as thirty-eight fungal genera in which Alternaria is the dominant genus. The distribution of genera in riping stage was more diversified than that in living stage. The inhibitory activity screening was conducted with fourteen species of microbes. The results showed that ninety-one strains, accounting for 95.8% of all the isolates, showed inhibitory activity to one indictor or more. Some endophytic fungi showed remarkable inhibitory activity to Escherichia coli CGMCC1.1103, Bacillus subtilis CGMCC1.769, Bacillus cereus ATCC1361, Enteropathogenic E. coli ATCC49105, Candida albicans ATCC10231, Pestalotiopsis microspora CNUMB2PM01. All fungal strains of endophytes obtained in this study showed no inhibitory activity to Staphyloccocus aureus CGMCC2.282 and Rhodotorula rubra ATCC12600.