一株海洋产电菌Shewanella sp. S2的筛选和产电分析

Isolation and Characterization of an Electricity-producing Strain Shewanella sp. S2 from Marine

摘要:

以厦门白城海域的潮间带表面沉积物为菌种来源筛选得到一株具有电催化活性的菌株S2, 该菌株的16S rRNA和gyrB基因发育树与Shewanella oneidensis MR-1同支, 相似性分别为98.5%和87%, 葡萄糖、木糖、半乳糖等碳源利用及最佳生长的NaCl浓度与S. oneidensis MR-1有显著差别, 因此初步鉴定为Shewanella 属菌株, 命名为Shewanella sp. S2。初步研究了菌株S2产电活性, 在以乳酸作为碳源产电时, 电压最高为150 mV, 相应的电流密度为66.1 mA/m2。

Abstract:

A new electrochemically active bacterium (exoelectrogen), strain S2, was isolated from coastal marine sediments of Xiamen. The phylogenetic trees based on 16S rRNA and gyrB gene sequences showed that strain S2 formed a lineage within the genus Shewanella and had high similarity (98.5% and 87.0%, respectively) with strain S. oneidensis MR-1. The phenotypic characteristics indicated strain S2 could be distinguished easily from S. oneidensis MR-1 by its culture conditions such as pH, NaCl tolerance and carbon source utilization. Based on these results, it is identified as Shewanella sp. S2. The preliminary current generation experiments showed that S2 could use lactate and xylose for electricity production. When lactate was used as a fuel, the MFC had highest voltage (150 mV) and current density (66.1 mA/m2).