产油微藻的分离、筛选及自养培养氮源、碳源的优化

Isolation, Selection of Microalgae for Lipid Production and Optimization of Its Nitrogen Resource and Carbon Resource in Autotrophic Culture

摘要:

从云南滇池的水样中分离筛选得到一株自养产油小球藻(Chlorella vulgaris, C. vulgaris), 其油脂产率可达28.6 mg/(L?d), 进一步考察了不同氮源、氮源浓度和添加无机碳源对其自养生长和油脂积累的影响。结果表明, 硝酸钠为优化氮源, 氮元素的优化浓度为123 mg/L, 油脂含量随氮元素浓度升高而降低; 添加NaHCO3显著提高了C. vulgaris生物量产率和油脂产率, 其优化浓度为800 mg/L。在氮源和碳源的优化浓度下, C. vulgaris的最大生物量产率和油脂产率可达332.8 mg/(L?d)和100.2 mg/(L?d), 分别是对照组的3.6和3.4倍。

Abstract:

An autotrophic oleaginous Chlorella vulgaris with lipid productivity at 28.6 mg/(L?d), was isolated from the water sample of Dianchi Lake in Yunnan province. The effects of nitrogen sources, nitrogen concentrations, and inorganic carbon (NaHCO3) on cell growth as well as lipid accumulation of C. vulgaris were investigated. The results showed that the optimum nitrogen source and nitrogen concentration were sodium nitrate and 123 mg/L, respectively. Lipid content decreased with the increasing of nitrogen concentration. Biomass productivity and lipid productivity of C. vulgaris remarkably rose by addition of NaHCO3. The optimized concentration of NaHCO3 was 800 mg/L. Under the optimal conditions, biomass productivity and lipid productivity of C. vulgaris could reach up to 332.8 mg/(L?d) and 100.2 mg/(L?d), respectively, which were 3.6 and 3.4 times as much as the control group.