Production and Characterization of Protease from Bacillus licheniformis in Solid-state Fermentation Using Deoiled Jatro-pha curcas Seed Cake as Substrate
1. Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, Guizhou, Guiyang 550003, China 在期刊界中查找 在百度中查找 在本站中查找
1. Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, Guizhou, Guiyang 550003, China 在期刊界中查找 在百度中查找 在本站中查找
1. Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, Guizhou, Guiyang 550003, China 在期刊界中查找 在百度中查找 在本站中查找
1. Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, Guizhou, Guiyang 550003, China 在期刊界中查找 在百度中查找 在本站中查找
1. Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, Guizhou, Guiyang 550003, China 在期刊界中查找 在百度中查找 在本站中查找
1. Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, Guizhou, Guiyang 550003, China 在期刊界中查找 在百度中查找 在本站中查找
1. Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, Guizhou, Guiyang 550003, China 在期刊界中查找 在百度中查找 在本站中查找
Solid-state fermentation was employed to produce protease by Bacillus licheniformis using deoiled Jatropha curcas seed cake as substrate. The optimal conditions of protease production were studied. Maximum protease production (7465 U/g of dry substrate, U/g) was obtained at 125% substrate moisture, a growth period of 3 days, supplementation with 10% (wt) of lactose and 5% (wt) of peptone. The results of protease analysis showed that the optimal temperature and pH were 55°C and 5?6, respectively. The Vmax and Km of protease produced by Bacillus licheniformis were 0.0324 μmol/(s?mg) and 0.0531 mmol/L, respectively. Organic solvent can enhance protease activity. In comparison with control, the activities were increased by 13.55% and 70.9% when the protease was solved by 10% (V/V) of methanol and 5% (V/V) of ethanol, respectively. Mg2+ enhanced protease activity by 42.54% while Hg2+ damaged protease and entirely inactive its activity.
