Rhizobium sp. N613胞外多糖的抗氧化活性

Antioxidant Activity of Exopolysaccharide from Rhizobium sp. N613

摘要:

以铁氰化钾和铁离子为检测系统测定了Rhizobium sp. N613胞外多糖(REPS)对氧化物的还原力; H2O2和Fe2+为羟自由基生成系统检测了REPS对羟自由基的清除作用; 邻苯三酚自氧化法检测了REPS对超氧阴离子的清除作用; 并体外检测了REPS对H2O2引起的氧化溶血现象的抑制作用及对小鼠肝组织脂质过氧化损伤的保护作用。结果显示, REPS对氧化物具有明显的还原力, 其对羟自由基的清除作用达到35.46%(多糖浓度为2 mg/mL), 对超氧阴离子的清除作用达到36.84% (多糖浓度为0.78 mg/mL), 对H2O2引起的氧化溶血现象的抑制作用达到43.84% (多糖浓度为1.14 mg/mL), 对小鼠肝组织脂质过氧化的保护作用达到34.46% (多糖浓度为1.14 mg/mL), 表明REPS具有良好的抗氧化作用, 有着重要的应用价值。

Abstract:

We investigated the reducing capacity of Caragana rhizobium exopolysaccharides (REPS) by the system of potassium ferricyanide and ferric chloride, determined the hydroxyl radical scavenging capacity of REPS by H2O2/Fe2+ system, measured the Superoxide radical scavenging capacity of REPS by the pyrogallol system, and we also determined its protective effects on H2O2 in hemplysis of erythrocytes and lipid per-oxidation of liver homogenate in rat. The results indicated that REPS has reductive capacity to oxides, the hydroxyl radical scavenging capacity of REPS was 35.46% (at the concentration of 2 mg/mL), the Superoxide radical scavenging capacity of REPS was 36.84% (at the concentration of 0.78 mg/mL), the protective effects of REPS on H2O2 induced hemolysis of rat erythrocytes was 43.84% (at the concentration of 1.14 mg/mL), and the protective effects on lipid per-oxidation of liver homogenate in rat was 34.46% (at the concentration of 1.14 mg/mL).