喜树内生放线菌多样性及抗菌活性评价

Diversity and Antimicrobial Activities of Endophytic Actinomycetes Isolated from Camptotheca acuminata Decne.

摘要:

从采集于云南大学的喜树中分离到了90株内生放线菌, 经16S rRNA基因序列分析鉴定, 分布于6个科10个属。对所有分离到的菌株进行抗菌活性检测, 发现33.4%的菌株有抗菌活性。通过PCR方法, 检测了PKSⅠ、PKSⅡ和NRPS基因, 阳性检出率分别为28.9%, 44.4%和48.9%。

Abstract:

Ninety endophytic actinomycetes were isolated from surface-sterilized samples of Camptotheca acuminata Decne. collected from Yunnan University. Results of 16S rRNA gene sequences analysis showed that they belong to 10 genera, 6 families. Among them, 33.4% of endophytic actinomycete cultures presented antimicrobial activity. The polyketide synthasesⅠ(PKS-I), polyketide synthases Ⅱ(PKS-II) and non-ribosomal peptide synthase (NRPS) sequences were respectively detected by PCR in 28.9%, 44.4% and 48.9% of tested strains.