Teachers were a focus of attention in traditional teaching mode, but students became the leading actor in constructivist teaching mode. There were many disadvantages in medical microbiology teaching, for example lesser hour, more information and lacking logicality, so the better teaching effect was not obtained in teaching medical microbiology with traditional teaching mode. We applied a theory of constructivism to medical microbiology teaching practice and designed constructivist teaching mode for medical microbiology teaching, which inspire students with go-aheadism and creativity. The teaching quality and students general ability can be improved with constructivist teaching mode in medical microbiology teaching.