By combining with curricular contents of microbiol ecology, optimized project integrating curricular emphasis and frontier of knowledge was set up according to the research progress on the mechanism of aerobic denitrification and the application of aerobic denitrifying bacteria in denitrification wastewater treatment process. As a prerequisite, to keep the teaching hours in classroom, method of extra-curricular studying based on curricular teaching improved the effectiveness of microbiol course by the example of de-nitrification teaching. This method is the effective way to the training of applied and innovative talents.