一种新香菇病毒基因组部分cDNA序列及病毒RT-PCR检测

The Partial Genome cDNA Sequence of a Novel dsRNA Virus from Lentinus edodes and the Virus Detected by RT-PCR

摘要:

本文报道从香菇菌丝体和子实体中分离到一种大小约20 nm × (100?200) nm的杆形病毒颗粒, 病毒基因组是大小约 8.0 kb 的dsRNA。对病毒基因组部分cDNA序列进行克隆, 完成1457 bp的核酸序列测定(Accession No: GQ372842), 该序列含1个不完整ORF, 编码314个氨基酸残基, 推测为病毒RNA聚合酶部分序列。病毒基因组部分cDNA序列与GenBank中的已知核酸序列无明显同源性, 表明它可能是新发现食用真菌病毒。为了对实验室和野外的香菇病毒进行快速检测, 我们根据得到的病毒基因组部分cDNA序列设计特异性引物, 建立了一种方便、有效检测香菇病毒的RT-PCR方法, 对感染病毒异常菌丝体中的病毒成功地进行了检测。

Abstract:

In this paper, a mycovirus was isolated from an edible mushroom, Lentinus edodes, in China. The virus particle is bacilliform with a size of 20 nm× (100?200) nm and contains a dsRNA genome about 8.0 kb. A fragment with 1457 bp of virus genome cDNA was cloned and sequenced (Accession No. GQ372842). This partial genome sequence has no obvious homology with known nucleic acid sequences in GenBank, which suggested that the virus may be a novel mycovirus. To detect the virus from Lentinus edodes in laboratory or field, we developed a convenient and effective RT-PCR method. With this method, the specific RT-PCR product was successfully amplified from the abnormal mycelia.