深圳赤潮中霍乱弧菌噬菌体的分离筛选及生物学特性分析

Isolation and Characterization of Bacteriophages of Vibrio cholera from the Red Tide Waters in Shenzhen

摘要:

本文以从鲍鱼肠道中分离出的霍乱弧菌SWBC-a为宿主菌, 采用双层平板法从2004年夏季深圳大梅沙海域赤潮海水样品中分离得到10株噬菌体。通过对分离出的噬菌体效价、RTD值的测定、形态特征的观察及对属内外细菌的交叉裂解谱的研究, 筛选出3株裂解谱较宽的强效噬菌体。选取其中2株高效裂解噬菌体PsaA和PsaH做生物学特性分析, 证明除镁离子有利于噬菌体裂解外, 温度、pH值、紫外照射、柠檬酸钠对噬菌体裂解都有不同程度的抑制。本研究结果为进一步利用噬菌体研发用于消除霍乱弧菌的微生态制剂和诊断食源性霍乱弧菌提供了理论基础。

关键词:噬菌体, 霍乱弧菌, 生物学特性, 理化因素

Abstract:

Using Vibrio cholerae seperated from the intestines of abalones as host, ten bacteriophages of Vibrio cholera were isolated by double-layer plate method from sea water sample from the Red tide at Dameisha of Shenzhen in 2004. Three broad lysis spectrum strains were found by detecting microbiological assay, RTD, the character of plaque morphology under electron microscope and the lysis spectrum. PsaA and PsaH were selected among all the strains as the representation to study the biological characteristics. The results proved that heat inactivation, pH, ultraviolet rays and sodium citrate restrict the ability of lysis to some dgree, additionally, magnesium perhaps have advantage on lysing. Furthermore, this study provide us further theory of researching on microbial ecological agents for eliminating Vibrio cholerae and diagnosising on food borne Vibrio cholerae using bacteriophage.

Key words:Bacteriophage, Vibrio cholerae, Biological characteristics, Physical and chemical factors