耐受有机溶剂洋葱伯克霍尔德菌ZYB002全细胞脂肪酶酶学性质

Properties of Whole-cell Lipase from Burkholderia sp. ZYB002 with Organic Solvent Tolerance

摘要:

一株对多种有机溶剂具有良好耐受能力的产脂肪酶菌株ZYB002经分子鉴定为洋葱伯克霍尔德菌。其产生的细胞结合脂肪酶最适温度为65?C, 最适pH为8.0, 在低于70?C和pH 3?8.5的范围内, 全细胞脂肪酶保持稳定。Ca2+、K+、Na+和NO3?等离子对脂肪酶活性有激活作用, 而Zn2+有抑制效应。全细胞脂肪酶对正丁醇有较强的耐受能力, 但曲拉通X-100对脂肪酶活性有强烈的抑制效应。洋葱伯克霍尔德菌ZYB002全细胞脂肪酶良好的碱稳定性、热稳定性和有机溶剂耐受性, 表明该全细胞脂肪酶具有重要的工业应用潜力。

关键词:洋葱伯克霍尔德菌, 有机溶剂耐受性, 全细胞脂肪酶, 酶学性质

Abstract:

The lipase-producing strain ZYB002 with broad-spectrum organic solvent-tolerance was identified as Burkholderia cepacia complex by the recA sequence. The optimal pH and temperature for lipolytic activity of the cell-bound lipase from Burkholderia sp. ZYB002 was 8.0 and 65°C, respectively. It was stable at temperature up to 70°C and retained 79.2% of its original activity for 1 h. The lipase was highly stable in the pH range from 3.0 to 8.5 for 6 h. Ca2+, K+, Na+, NO3?, etc. ions stimulated its lipolytic activity, whereas Zn2+ ions caused inhibition. The cell-bound lipase was also relatively stable in n-butanol at a final concentration of 50% (V/V) for 24 h. However, the lipase was strongly inhibited in triton X-100 at a final concentration of 10% (V/V). The cell-bound lipase with thermal resistance, alkaline resistance and organic solvent resistance showed its’ great potential in various industrial application fields.

Key words:Burkholderia sp., Organic solvent tolerance, Whole-cell lipase, Biochemical characterization