一种简化的巴斯德毕赤酵母基本培养基

A Simplified Minimal Medium for Pichia pastoris

摘要:

通过逐步减少巴斯德毕赤酵母基本培养基中生长因子和微量元素的组成种类, 最终确认维持巴斯德毕赤酵母在平板上的生长, 除生物素外其它生长因子和微量元素不必额外添加。据此提出了一种简化的巴斯德毕赤酵母基本培养基SPMD, 其成分为6种无机盐、生物素和葡萄糖。除葡萄糖单独灭菌外, 配制时可以高温高压灭菌。使用该培养基用于转化子的筛选实验中, 获得了与使用常规MD培养基基本相当的转化效率。

Abstract:

In this study, by gradually reducing the kinds of the trace elements and growth factors from the minimal medium of Pichia pastoris, the result showed that to maintain the normal growth of P. pastoris on agar plate, trace elements and growth factors except biotin don’t need to be added specially. A simplified minimal medium of P. pastoris, SPMD was presented, containing six kinds of inorganic salt, biotin and glucose. All components can be sterilized by autoclave except glucose which should be sterilized separately. This medium was used in screening for transformants and got a transformation rate comparable to that using MD medium.