基因工程FR-008/杀念菌素脱羧衍生物CS103的分离提取及毒性和抗白色念珠菌活性研究

Research on the Isolation, Cell Toxicity and Antifungal Activity Against Candida albicans of Novel Decarboxylated FR-008/Candicidin Derivative CS103

摘要:

本文在建立了基因工程FR-008/杀念菌素脱羧衍生物CS103分离提取工艺的基础上, 经进一步纯化, 获得一定供试样品。通过对比脱羧FR-008/杀念菌素CS103、FR-008/杀念菌素和两性霉素B三种化合物对人胚肾细胞毒性、对人血红细胞的溶血活性和对白色念珠菌的抗菌效果, 发现脱羧FR-008/杀念菌素CS103的毒性较FR-008/杀念菌素和两性霉素B大大降低, 且保持了较高的抗真菌(Candida albicans)活性。

Abstract:

Based on founding the methods for isolation and purification of the novel decarboxylated FR-008/Candicidin derivative CS103, we obtained enough samples for testing from the culture mycelia of the mutant of Streptomyces FR-008. Through comparing the cell toxicities on Human Embryonic Kidney Cells 293, haemolytic activities on human erythrocytes and antifungal activities on C. albicans, we found that the toxicity of decarboxylated FR-008/Candicidin derivative CS103 had been lower than FR-008/Candicidin and Amphoteicin B, while it still had high antifungal activity on C. albicans.