The effects of the crude toxin of Fusarium oxysporum f. sp. cubense race 4 (Foc 4-zj) on the growth of Bacillus licheniforms R21 strain and its protein secretion were studied. The results showed that the growth of R21 strain was inhibited by the crude toxin produced by Foc 4-zj, and its growth cycle was shorten. The protein contents were decreased and the kinds of protein in the supernatant fluid had changed significantly after treated with crude toxin. High dose of the crude toxin greatly decreased the contents of antagonistic proteins, whereas lower one increased.