阪崎肠杆菌显色培养基的应用研究

Application of Enterobacter sakazakii chromogenic medium

摘要:

阪崎肠杆菌(Enterobacter sakazakii)是新近引起广泛关注的一种危险的条件致病菌, 主要存在于婴幼儿奶粉、婴幼儿补充食品中。由于目前日常使用的传统检验方法存在检测周期长等方面的不足之处, 本实验室研究设计出一种新的显色培养基(HKMCES), 通过与OXOID公司的同类产品(OXCES)比较, 分别应用于质控菌株、污染样品和实际样品的测试, 对这2种显色培养基的灵敏度、特异性、检测效果以及前增菌方法进行了初步评价。结果表明, 合适的增菌方法更有利于样品中阪崎肠杆菌的检出, 本实验室研制的显色培养基和OXOID公司的显色培养基均具有较好的选择性和特异性, 检测效果相当。这种新的显色培养基能使检测周期缩短, 具有较好的应用价值。

Abstract:

Enterobacter sakazakii is an opportunistic pathogen which has been isolated at low levels from powdered infant formulas. As there are deficiencies as more time in the general method of isolation and identification of Enterobacter sakazakii, a new chromogenic medium(HKMCES) was designed in this a assay. Through detecting the reference strains, artificeially contaminated samples and natural samples, the sensitivity, specificity, detection effect and the way of pre-enrichment of HKMCSE were studied and compared with OXCSE. The results showed that the isolation of E. sakazakii was more efficiency after adequate pre-enrichment was applied. HKMCSE had the same selectivity and highly specificity as OXCSE. In con-clusion, Enterobacter sakazakii chromogenic medium were invaluable which may shorten the detection pe-riods.