细菌测试片的冷水可凝凝固剂初步研究和应用

Preliminary Study and Application on Cold Water Gelling Agent for Bacterial Testing Slip

摘要:

微生物测试片具有携带方便、不用配制培养基的优点, 为一种新型的检测工具。测试片的技术关键是冷水可凝胶, 本文通过研究新的冷水可凝凝固剂(HKG)的保水性能、pH、强度、微生物利用及降解和抑菌效果, 再组装成细菌菌落总数测试片, 进行特异性和食品样品效果检验, 并与倾注平板法比对, 评价其作为微生物测试片凝固剂的可行性。结果显示HKG对16种微生物均无抑菌作用, pH为中性, 强度为340.31 g/cm2 ? 48.71 g/cm2; 具有良好的保水性能; HKG在短时期(5 d)内不支持微生物生长, 不被分解利用, 在测试片和倾注培养法中5种测试菌株均可达相同的灵敏度(P = 0.438 > 0.05), 各菌株菌落数(P = 0.442 > 0.05)和食品样品菌落数(P = 0.718 > 0.05)比较在统计学上无显著差异。结果表明, HKG能较好地应用于细菌测试片计数, 并能提高检测效率。

Abstract:

Microbial testing slip is a new detection tool which is convenient to carry and free of media preparation. Since the key technique of the testing slip is cold water gelling agent, a new cold water gelling agent (HKG)’s water retention capacity, pH value, gels strength, source utilization by microorganisms and inhibition effect were tested in this assay. Furthermore, detection effect and specificity of HKG total plate count bacterial testing slip of food samples were compared with pour-plate method in order to evaluate its feasibility of gelling agent in microbial testing slip. HKG showed no antibacterial effect to 16 kinds of microorganisms, also the features of neutral pH, gels strength of 340.31 g/cm2 ± 48.71 g/cm2, good water retention capacity, HKG can not support the growth of microbe or be degrading by microorganism in short time (5 d). HKG testing slip had the same sensitivity (P = 0.438 > 0.05) as pour plate method in detection of 5 strains, the colony count of various test strains (P = 0.442 > 0.05) and the colony count of food samples (P = 0.718 > 0.05) of HKG testing slip had no significant difference in statistics compared with pour plate method. As the gelling agent, HKG shows good performance in bacterial testing slip, and can also improve the detection efficiency.