猪链球菌2型菌毛样结构蛋白SSU2101原核表达及其免疫保护性

Expression of Pili-like Protein SSU2101 of Streptococcus suis Serotype 2 and Evaluation of Its Protective Abilities to a Mouse Model

摘要:

为了研究猪链球菌2型(Streptococcus suis serotype 2, S. suis 2) 05ZYH33株预测的菌毛样结构蛋白(Pili-like protein, PLP) SSU2101的免疫保护性作用, 本试验通过PCR扩增出plp基因片段, 进一步将目的基因克隆到表达载体pET32a中, IPTG诱导重组蛋白表达, 亲和层析法纯化目的蛋白。Western blot分析表明该重组蛋白具有良好的免疫原性, 动物试验结果证实PLP蛋白对S. suis 2强致病株感染小鼠具有显著的免疫保护作用, 提示菌毛样结构蛋白SSU2101是理想的猪链球菌2型亚单位疫苗的候选分子。

Abstract:

To prokaryotic express pili-like protein(PLP) SSU2101 of the Chinese strain 05ZYH33 of Streptococcus suis serotype 2(S. suis 2), elucidate its immunogenicity and immunoprotection. The plp gene was amplified by PCR, and then cloned into prokaryotic expression plasmid pET32a. The recombinant plasmid was transformed into E. coli BL21 competent cells to express the recombinant PLP(rPLP). The rPLP protein was purified by affinity chromatography methods. Western blot indicated clearly that the rPLP shares strong specific immunogenicity. Animal infection experiments demonstrated that the rPLP protect mice from lethal challenge with S. suis 2 infection, which suggest rPLP might be a candidate component of S. suis 2 protein vaccine.