药用植物内生放线菌的分离、筛选及活性菌株YIM 61470鉴定

Endophytic Actinomycetes Associated with Medicinal Plants: Isolation, Antimicribial Activity and Identification

摘要:

从云南西双版纳热带雨林多种药用植物中分离到272株内生放线菌, 活性筛选表明146株菌的发酵产物具有抗菌活性, 其中94株菌具有拮抗病原细菌活性, 127株菌具有抑制病原真菌的功能。分离菌株YIM 61470具有广谱抗菌活性, 通过形态特征、培养特征、生理生化特征、细胞化学分类特征和基于16S rRNA基因序列的相似性分析等研究, 菌株YIM 61470被鉴定为链霉菌属(Streptomyces)氢化链霉菌(S. hydrogenans)的一个菌株。

Abstract:

272 endophytic actinomycetes were isolated from different medicinal plants collected from tropical rainforest in Xishuangbanna of Yunnan province. By antimicrobial screening, the fermentation broth of 146 strains inhibited the tested pathogenetic strains, and 94 or 127 out of the 272 endophytic actinomycetes strains showed inhibition activity against pathogenetic bacteria or fungi. The strain YIM 61470 exhibited extensive antagonism against pathogenic microbes. Based on the morphological characteristics, cultural characteristics, physiological characteristics, chemotaxonomic data and 16S rRNA gene sequence similarity analysis, strain YIM 61470 was identified as Streptomyces hydrogenans, a known species of the genus Streptomyces.