白桦茸凝集素提取工艺的优化

Optimization of Extraction Process About Lectin from Phaeoporus obliquus(Pers ex Fr).J.Schroet

摘要:

对白桦茸凝集素最佳提取工艺进行了研究。以2%兔血细胞凝血效价为指标, 确定了最佳提取缓冲液。通过正交试验对料液比、提取时间、提取液pH值、NaCl浓度等因素进行了优化分析并确定了提取工艺的最佳参数组合。结果表明, 以TBS和PBS为提取缓冲液所得的白桦茸凝集素凝集效价分别为64、16; 最佳提取工艺: 液料比为50:1, 提取时间为20 h, NaCl浓度为0 mol/L, 缓冲液pH值为8.0, 按该最佳工艺提取白桦茸凝集素凝血效价为256。所优化的提取工艺稳定、可行, 为该凝集素进一步在免疫调节方面的开发应用提供一定基础。

Abstract:

This paper described the results of the optimal method for preparation and extraction of lectin from Phaeoporus obliquus(Pers ex Fr).(J.Schroet). We determined the optimal buffer solution using the ag-glutination test with 2% hare blood cells. The optimal extraction process included the following parameters that were conformed by optimization analysis: material-water ratio, extraction time, concentration of sodium chloride and the pH value. The results are that the agglutination indexes of POL using TBS and PBS extrac-tion buffer were 64 and 16, respectively. The optimal extraction process is that, the material-water ratio was 1:50, the extraction time was 20 h, while the concentration of sodium chloride was 0 mol/L and pH was 8.0. The agglutinability of POL was 256 examined by this method. The optimized extraction process is stable and practicable, which may supply some basis for the application of the lectin from Phaeoporus obliquus in immunoregulation.