仿刺参“腐皮综合症”病灶处优势菌的分离鉴定及AHLs信号分子的检测

Identification and AHLs Detection of Dominant Bacterium of Sea Cucumber (Apostichopus japonic) Infected with the Skin Ulceration Syndrom

摘要:

仿刺参“腐皮综合症”是一种由细菌引起的高传染性、高死亡率疾病。为了研究仿刺参“腐皮综合症”病灶处优势菌以及其是否存在N-酰基高丝氨酸内酯类化合物(AHLs)介导的群体感应系统, 本文从患病仿刺参病灶处分离纯化出7株优势菌, 生理生化指标测定和16S rDNA序列分析表明: 菌株C6属Tenacibaculum属, 菌株4属于腐败希瓦菌群(Shewanella putrefaciens group), 菌株TB属于弧菌属(Vibrio), 菌株BP2、BP3、BP4及BP6属于假交替单胞菌属(Pseudoalteromonas)。采用AHLs的高效检测菌株根癌农杆菌(Agrobacterium tumefaciens KYC55)对优势菌的AHLs活性进行检测, 其中菌株C6、4、TB、BP3及BP4存在以AHLs为信号分子的群体感应系统, 菌株BP2与BP6则无AHLs活性; 不同细菌AHLs活性不同, AHLs活性从高到低顺序为4>TB>BP4>BP3>C6。

Abstract:

The skin ulceration syndrome of sea cucumber is a kind of desease induced by bacterium. In order to investigate the bacterium of infected sea cucumber and detect the N-acyl-homoserine lactones(AHLs) se-cretion of the bacterium, 7 bacterial strains were isolated from the infected sea cucumber. These strains were identified by physiological-biochemical characteristics and 16S rDNA sequence. Results show that strain C6 belongs to Tenacibaculum, strain 4 belongs to Shewanella putrefaciens group, strain TB belongs to Vibrio, strain BP2, BP3, BP4 and BP6 belong to Pseudoalteromonas, respectively. AHLs were detected with strain Agrobacterium tumefaciens KYC55. Among these bacterial strains, strain C6, 4, TB, BP3 and BP4 can se-cret AHLs, while strain BP2 and BP6 can’t. And the AHLs activity differs, from the highest to the lowest are 4, TB, BP4, BP3 and C6.