星形孢菌素产生菌H41-38的发酵工艺条件

Fermentation Conditions of Producing Staurosporine Strain H41-38

摘要:

本研究对菌株H41-38发酵生产星形孢菌素的工艺进行了研究。结果表明: 30?C、96 h为菌株摇瓶培养最适条件。玉米淀粉和酵母粉为最适碳、氮源。采用Fractional factorial design方法确定培养基成分的重要因子: 酵母粉和粗盐, 然后利用爬坡路径试验和响应面分析法优化了发酵效价取得最大值时酵母粉和粗盐含量, 发酵效价最大理论值为252.31 ?g/mL, 摇瓶实测值为251.28 ?g/mL, 其发酵水平比优化前提高了4.33倍。在30 L发酵罐(300 r/min、30?C、V空气=10 L/min~15 L/min)中扩大培养84 h后发酵效价达到286.44 ?g/mL, 比摇瓶培养效价提高了45.16 ?g/mL, 其发酵参数变化曲线可指导生产罐的应用生产。

Abstract:

This paper focused on stausporine production by cultivation of strain H41-38. Results could be summarized as follows. The optimal condition for the shake flask cultivation for this strains was 30°C、96 h. Results indicated that the best carbon and nitrogen sources were cornstarch and yeast powder. With the method of fractional factorial design, it was found that yeast powder and crude salt were the important factors, and their optimal contents were determined. Under the optimal conditions, the calculated maximal stausporine was 252.31 μg/mL, while the experimental value of shake flask was 251.28 μg/mL. The titer was 4.33 times more than using the original culture medium. After cultivating in a 30 L fermentor (300 r/min、30°C, Vair=10 L/min~15 L/min) for 84 h, the titer was 286.44 μg/mL, which titer was 45.16 μg/mL higher than the shake flask cultivation, and curves of parameter provided useful information and data for the industrial production of stausporine.