低温纤维素酶菌株CNY086选育及发酵培养基优化(Ⅱ)

Breeding and Fermentation Medium Optimization of-Cold-active Cellulase Strain CNY086 (Ⅱ)

摘要:

自渤海湾海泥中分离21株低温纤维素酶产生菌。其中菌株CNY01为绿色木霉(Trichoderma viride), 酶活力为67.30 U/mL。以该菌株为出发菌株, 经UV、DES等诱变, 选育出高产突变菌株CNY086, 酶活力为92.17 U/mL。该突变菌株低温纤维素酶发酵具有遗传稳定性。通过单因素和正交实验确定突变菌株CNY086低温纤维素酶发酵最适培养基: 秸秆粉1.20%、麸皮0.70%、硫酸铵0.50%、磷酸二氢钾0.55%, 上述条件下CNY086菌株酶活力达到108.55 U/mL。

Abstract:

21 cold-active strains which produced cold-active cellulase were isolated from Bohai Bay. One of the strains CNY01 was a kind of Trichoderma viride and its cellulase activity was 67.30 U/mL. Through UV and DES mutation, the strain CNY086 was bred and its cellulase activity was 92.17 U/mL. The strain CNY086 had stable ability to produce cellulase. Through orthogonal experiment, the optimal condition of the fermentation medium had been tested. The suitable medium was that straw power 1.20%, bran 0.70%, (NH4)2SO4 0.50%, KH2PO4 0.55%, in this condition the cellulase activity came to 108.55 U/mL.