古银杏内生真菌的分离及其抑菌活性

Isolation and Antimicrobial Activity of Endophytic Fungi from Ginkgo biloba L.

摘要:

采用组织分离法从古银杏健康组织中分离得到55株内生真菌, 其中28个分离菌株在PDA培养基上不产孢子, 占总分离菌株的50.9%, 其它菌株根据其在PDA培养基上的培养特征, 10株被鉴定为青霉、6株为曲霉、4株为交链孢霉、3株为简梗孢霉, 另外酵母、毛霉、小单头孢霉、镰孢霉各1株。考察内生真菌培养上清对7种受试指示菌的抑制作用, 共筛选得到23株至少对一种指示菌的生长有抑制作用的菌株, 其中11株为不产孢真菌, 占活性菌株的47.83%。对活性最强的一株菌进行形态学和分子生物学鉴定, 将其确定为Xyla

Abstract:

Endophytic fungi inhabited in Ginkgo biloba L. were isolated, and 55 endophyte strains were obtained. Among the strains, 28 produced sterile mycelia on PDA plates, accounting for 50.9% of the total fungi. In addition, there were ten Penicillium spp., six Aspergillus spp., four Alternaria spp. and three Chromosporium spp., and one yeast, Mucor, Acremoniella, and Fusarium, respectively. Antimicrobial activity of fermentation cultures of these 55 strains were investigated against seven tested microorganisms. Twenty-three strains with antimicrobial activity were obtained, among which eleven produced sterile myce-lia, accounting for 47.83% of the fungi with antimicrobial activity. The strain with the highest antimicrobial activity was determined to be Xylaria venosula Speg., according to its morphological features and molecular analysis. As a novel antimicrobial resource, endophytic fungi in G. biloba have a potential prospect.