With the development of the food industry in China, it has been found that food safety is becoming the biggest issue in the food manufacture and logistics. Accurate and timely to establish a risk assessment method in produce market is the challenge for food safety system. Predictive microbiology is a core early warning technology in the food safety risk assessment. According to the microorganism predicting model, the pathogen and spoilage microorganism’s growth in food can be fast judgment in advance. And it plays an important part in controlling the growth of pathogen and the spoilage microorganism in food. This paper summarized the predictive microbiology model’s establishment and the present research situation, and discussed the present situation and application of predictive microbiology in food safety risk assessment. The future trend of predictive microbiology in food safety risk assessment was prospected as well.