新的产弹性蛋白酶菌株的筛选与鉴定

Screening and Identification of a New Elastase-producing Strain

摘要:

本研究从秸秆中分离得到一株弹性蛋白酶高产菌, 并对该菌株进行鉴定, 以期为实现其工业化生产提供理论依据。采用酪蛋白(脱脂奶粉)进行初筛后, 以弹性蛋白(牛筋)进行复筛, 并对筛选结果进行检验, 然后对所得菌株结合形态、生理生化特性和16S rRNA基因序列进行分类鉴定, 得到一株弹性蛋白酶高产菌株LSF-97。结果显示, 菌株LSF-97与短小芽孢杆菌同源率达到100%, 形态及生理生化特征与模式菌也显示高度一致性, 故将其确定为短小芽孢杆菌。

Abstract:

The study provide a theoretical basis for the industrial production of a elastase-high-yield strain which was isolated from the straw, and the selected strain was identified. The screening strategy included casein (skim milk) plate selecting and elastin (beef tendon) re-screening. And then, the morphological, physiological and biochemical characteristics as well as 16S rRNA sequence homology of the selected strain were studied. Finally, the strain LSF-97 which had a excellent decomposing ability to beef tendon was obtained. The results showed that the strain LSF-97 is relative to the Bacillus pumilus with 100 % similar in sequence under the phylogenetic tree, the morphological and physiological and biochemical characteristics are also consistent with pattern of bacteria. So it was identified as Bacillus pumilus.