辐射污染区土壤中放线菌的分离及多样性

Diversity Investigation of Actinomycetes Isolated from Radiation-polluted Soil

摘要:

从辐射污染区采集42份土样, 分别采用6种分离培养基进行放线菌的分离, 共获得152株放线菌。经形态、核糖体DNA扩增片段限制性内切酶(Amplifed ribosomal DNA restriction analysis, ARDRA)分析比较, 选取其中的60株进行16S rRNA基因测序。通过序列比对、聚类分析, 60株菌分布在放线菌纲中的12个属, 链霉菌属占大多数, 有大量稀有放线菌, 这表明辐射污染区具有较丰富的放线菌属种多样性。

Abstract:

One hundred and fifty two actinomycetes were isolated from forty two radiation-polluted soil samples, using six different isolation media. Sixty cultures were chosen for 16S rRNA gene sequence and systematic analysis, which based on their morphology and ARDRA. Results of 16S rRNA gene sequences blasting showed that the strains were assigned to 12 recognized genera of actinomycetes, most of them fall within Streptomyces genus and a great deal of strains belonged to rare actinomycetes, which indicated a rich diversity of actinomycetes in the radiation-polluted soil.