胶毒素与BSA的相互作用

Study on the Interaction of Gliotoxin with BSA

摘要:

应用荧光、圆二色和紫外—可见吸收等波谱法研究胶毒素与牛血清白蛋白(BSA)的相互作用。荧光光谱实验结果表明胶毒素主要靠疏水作用与BSA结合, 而对其内源荧光产生猝灭作用,其淬灭方式为静态猝灭, 胶毒素与BSA的结合常数为7.2×103 L/mol。圆二色光谱检测发现, 随着胶毒素浓度的增加, BSA的a-螺旋数量也增加, 当胶毒素浓度为BSA浓度的100倍时, BSA的a-螺旋增加40.1%, 表明胶毒素与BSA的结合改变了BSA的空间构象。

关键词:胶毒素, 荧光光谱, 圆二色光谱, 紫外—可见吸收光谱

Abstract:

The interaction between Gliotoxin and bovine serum albumin (BSA) was studied by the fluorescence, Circular Dichroism (CD) and ultraviolet visible (UV-Vis) techniques. The fluorescent experiment showed that the intrinsic fluorescence of BSA was quenched by the binding of gliotoxin in a static quenching procedure, with an association constant of 7.2′103 L/mol and in hydropobic forces. And the CD spectrum revealed that gliotoxin effected the conformation of BSA by increased the mass of α-helix.

Key words:Gliotoxin, Fluorescent spectrum, CD specrum, UV-Vis spectrum