科微学术
微生物学通报
2025年4月21日 周一
东北农业大学创新团队基金(No. CXT003-1-3); 黑龙江省教育厅科学技术研究项目(No. 11541015); “十一五”国家科技支撑计划项目(No. 2007BAD87B03)
LI Chun-Yan
College of Resource and Environment, Northeast Agricultural University, Harbin, Heilongjiang 150030, China; Post-doctoral station of Northeast Forestry University, Post-doctoral workstation of Heilongjiang Academy of Agricultural Science, Harbin, Heilongjiang 150001, China利用富集培养技术从长期施用丁草胺的稻田土壤中分离得到能够降解丁草胺的细菌1株, 标记为LYC-1。经形态特征、生理生化特征和16S rRNA序列分析, 将该菌株鉴定为不动杆菌属(Acinetobacter sp.), 菌株LYC-1的最适生长温度为30°C, 最适pH值为7.5。当接种量为5%时, 该菌株在含100 mg/L 的丁草胺无机盐基础培养液中培养7 d后, 可使丁草胺降解达80%以上。
One Butachlor-degrading bacterium named LYC-1 was isolated from a soil sample by enrichment culture. Combining morphologic characteristics and physiobiochemical characteristics with 16S rRNA sequence analysis, strain LYC-1 was identified as Acinetobacter sp.. The optimal temperature and pH value for growth of strain LYC-1 was 30°C and pH 7.5 , respectively. The strain could degrade butachlor more than 80% when it was cultured in 100 mg/L butachlor medium for 7 days.
李艳春,熊明华,肖 晶,李春艳. 一株丁草胺降解菌的分离鉴定及其降解特性的研究[J]. 微生物学通报, 2009, 36(8): 1178-1182
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