黑曲霉B0201利用五倍子固体发酵产单宁酶的条件研究

Research on Conditions of Tannase Production from Aspergillus Niger B0201 Under Solid-state Fermentation Using Gallnut

摘要:

通过比较常规灭菌发酵和生料发酵, 研究黑曲霉B0201利用五倍子固体发酵产单宁酶的条件。结果表明, 在生料发酵过程中, 采用20%五倍子并且以(NH4)2SO4为氮源制备单宁酶的最佳条件为: 液固比=1.6:1、温度30°C、初始pH 6.0。在该条件下通过96 h的培养, 单宁酶的活力可达51.2 U/gds, 是常规灭菌发酵的3.6倍。以上结果显示, 生料发酵生产单宁酶是一种高效可行的方法。

Abstract:

According to the comparative research of conventional sterile fermentation and uncooked material fermentation, the conditions of tannase production from Aspergillus Niger B0201 under solid-state fermentation using gallnut was studied. The results showed that the liquid?solid ratio of 1.6: 1, temperature of 30°C, initial pH of 6.0 were the optimal conditions for tannase production under uncooked material fermentation with gallnut content of 20% and (NH4)2SO4 for nitrogen sources. Under this condition, tannase activity reached to 51.2 U/gds after 96 h of cultivation, which was 3.6 times as the conventional sterile fermentation. The results above revealed that under uncooked material fermentation for tannase production was a feasible method with high efficiency.