Brevibacillus brevis HOB1所产脂肽的分离纯化和鉴定

Isolation, Purification and Identification of a Lipopeptide Produced by Brevibacillus brevis HOB1

摘要:

用常压反相色谱对从短短芽孢杆菌HOB1发酵液中提取到的脂肽类生物表面活性剂进行了分离纯化, 并用HPLC制备了其中的一个化合物。经电喷雾质谱分析得到该化合物的相对分子量为1035.7 D, GC/MS的分析结果显示其脂肪酸部分为C15 β-羟基脂肪酸, 由PITC柱前衍生法测得该化合物的氨基酸组成比例为: Asp:Glu:Val:Leu = 1:1:1:4。结果显示该脂肽的结构与表面活性素(C15 surfactin)类似。实验表明, 除芽孢杆菌属外, surfactin系列脂肽还能为短芽孢杆菌属所产生。

Abstract:

The lipopeptide samples were purified by normal reverse-phase column chromatography after isolated from the cell free broth of Brevibacillus brevis HOB1. A lipopeptide was separated from these samples by preparative HPLC, and determined to have a molecular weight of 1035.7 D by ESI-MS. The fatty acid part was identified to be C15 β-hydroxyl fatty acid by GC/MS. A quantitative analysis of the PITC-derivated amino acids of the lipopeptide by HPLC gave the following molar ratios: Asp 1, Glu 1, Val 1, Leu 4. All the results indicated that the lipopeptide has a structure similar to C15 surfactin. This study revealed that surfactin series lipopeptides can be produced by Brevibacillus genus in addition to Bacillus genus.