Key Laboratory of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China 在期刊界中查找 在百度中查找 在本站中查找
Key Laboratory of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China 在期刊界中查找 在百度中查找 在本站中查找
Key Laboratory of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China 在期刊界中查找 在百度中查找 在本站中查找
A biosurfactant producing bacterial strain C-3 was isolated from the sludge of a petroleum refinery and identified as Pseudomonas aeruginosa. The optimal conditions for the growth and biosurfacant production of strain C-3 were investigated. The results showed that strain C-3 could grow well and produce the largest amount of biosurfacant under the following conditions: seed-oil as sole carbon source, 30°C, initial pH 8, Ca2+ concentration of 20 mg/L, 75 mL medium in 250 mL Erlenmeyer flask. The biosurfactants produced by strain C-3 was identified as lipopeptides, showing high surface activity and emulsifying efficiency with critical micelle concentration (CMC) being 50 mg/L.
