三种金黄色葡萄球菌显色培养基检测效果的比较

Comparison of the Detection Effect of Three Chromogenic Media of Staphylococcus aureus

摘要:

本文研究2种国外主流的金葡菌显色培养基和广东环凯微生物科技有限公司生产的PEN-TCF检测金葡菌的效果。通过研究3种产品对多种金葡菌菌株的回收率、最低检出限以及检测人工污染食品的回收率、特异性和抗干扰能力比较其检测效果。结果表明3种培养基的最低检出限相近, 显色培养基A和PEN-TCF的回收率较高, 但抗干扰能力较低; 显色培养基B回收率稍低, 而抗干扰能力较强; PEN-TCF和培养基B的特异性更优于培养基A。

Abstract:

Two current main chromogenic media products of abroad and the newly developed PEN-TCF from Guangdong Huankai Microbial Sci. & Tech. Corporation were compared in this study. Their recovery of different Staphylococcus aureus strains, minimum detection limits and the recovery, specificity and anti-distubance of detecting artificial contaminated samples were studied. The results showed that 3 media had similar minimum detection limits, Chromogenic Media A and PEN-TCF had better sensitivity but worse anti-distubance, while Chromogenic Media B had better anti-distubance yet lower sensitivity, and Media B and PEN-TCF were more specific than Media A.