Biolog EcoPlateTM is a mainstream approach of studying community-level physiological profiles of microbes. To correct misunderstanding and insufficiency of experimental result explanation, we announced an amendment of information extraction of Biolog EcoPlateTM, which included two parts: (1) carbon substrates assortment sheets based on microbial physiological metabolism and ecological function, providing referrence and index for researchers; (2) illustration of how to use assorment sheet and ordination method to find out more information, guiding by environmental concentration principle.