Biolog EcoPlateTM实验信息提取方法改进

An Amendment on Information Extraction of Biolog EcoPlateTM

摘要:

Biolog EcoPlateTM微平板是用于研究微生物群落水平多样性的一种主流研究方法。针对目前国内研究者对Biolog实验结果解读不充分和错误层出不穷的情况, 提出了一套改进实验结果信息提取的方法。方法包含两个部分, 一是基于微生物生理学和生态学意义上的Biolog碳源分类表, 是研究者进行解读的参考依据和索引; 二是结合实例, 阐释如何根据上述分类表、以环境富集原理为指导, 利用排序的统计方法进行结果解译, 发掘出更多的信息。

关键词:Biolog, 群落水平多样性, 碳源, 分类, 解读

Abstract:

Biolog EcoPlateTM is a mainstream approach of studying community-level physiological profiles of microbes. To correct misunderstanding and insufficiency of experimental result explanation, we announced an amendment of information extraction of Biolog EcoPlateTM, which included two parts: (1) carbon substrates assortment sheets based on microbial physiological metabolism and ecological function, providing referrence and index for researchers; (2) illustration of how to use assorment sheet and ordination method to find out more information, guiding by environmental concentration principle.

Key words:Biolog EcoPlate, Community-level physiological profiles, Carbon substrates, Classification, Explanation