沙门氏菌中dnd基因簇的缺失与异源表达

Deletion of dnd Gene Cluster in Salmonella enterica and Heterogenous Expression

摘要:

沙门氏菌Salmonella enterica serovar Cerro 87是一株从鸡场分离到的菌株, 其DNA骨架上的磷硫酰化导致了在高压脉冲电泳过程中DNA降解(DNA degradation, Dnd表型)。本研究采用SacB所介导的负筛选系统, 在该菌株中成功缺失了dnd基因簇, 构建了突变株XTG103, 该突变株不再具有Dnd表型。通过异丙基-b-D硫代半乳糖苷(IPTG)诱导启动子PlacZ的转录可以调控DNA磷硫酰化修饰的dnd基因簇的异源表达。

Abstract:

Salmonella enterica serovar Cerro 87, which was isolated from a commercial egg-producing farm, has a phosphorothioated DNA backbone resulting DNA degradation(Dnd) during the