一种双歧杆菌胞外多糖免疫调节功能研究

Study on Immunoregulation Effect of Exopolysaccharide Produced by Bifidobacterium spp.

摘要:

根据保健食品功能评价规范?功能学评价程序研究一种双歧杆菌胞外多糖(Bifidobacterium spp. exopolysaccharide, EPS)的免疫调节功能。通过脾淋巴细胞增殖反应、绵羊红细胞诱导小鼠迟发型变态反应(DTH)、血清溶血素测定以及巨噬细胞吞噬实验探讨该EPS的免疫调节活性。EPS分别以高、中、低剂量组连续口服给药10天, 结果发现低剂量的EPS[100 mg/(kg·d)]可以促进脾淋巴细胞增殖; 高、中、低剂量对绵羊红细胞诱导的小鼠迟发型变态反应均无促进作用; 但是, EPS 高、中、低剂量组均能明显提高小鼠血清半数溶血值HC50以及增强小鼠腹腔巨噬细胞吞噬鸡红细胞的吞噬能力。根据规范可知, 该双歧杆菌EPS 具有一定的免疫调节活性。

Abstract:

The immunoregulation effect to polysaccharides from Bifidobacterium spp. was investigated on the base of functional assessment standards of health food. Effects of the EPS on immunity were investigated by promoted the proliferation of spleen lymph cells, delayed type hypersensitivity reaction, the HC50 value and macrophage function assay in mice. Data showed that the EPS could obviously increase the ratio of swallowed chicken red blood cell by macrophage and the HC50 value in mice. However, no significant effect was found on the delayed type hypersensitive induced by sheep red blood cell, for only the low dose of 100 mg/(kg·d) EPS promoted the proliferation of spleen lymph cells. Bifidobacterium spp. EPS can certain immunomodulating function.